consensus irf 1 gel shift oligonucleotide (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
consensus irf 1 gel shift oligonucleotide
Consensus Irf 1 Gel Shift Oligonucleotide, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/consensus+irf+1+gel+shift+oligonucleotide/IRF-1+Gel+Shift+Oligonucleotides/pmc01808467-73-21-26
Average 93 stars, based on 25 article reviews
Consensus Irf 1 Gel Shift Oligonucleotide, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/consensus+irf+1+gel+shift+oligonucleotide/IRF-1+Gel+Shift+Oligonucleotides/pmc01808467-73-21-26
Average 93 stars, based on 25 article reviews
consensus irf 1 gel shift oligonucleotide - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Distinct mechanisms of loss of IFN-gamma mediated HLA class I inducibility in two melanoma cell lines"
Article Title: Distinct mechanisms of loss of IFN-gamma mediated HLA class I inducibility in two melanoma cell lines
Journal: BMC Cancer
doi: 10.1186/1471-2407-7-34
Figure Legend Snippet: Time course effect of IFN-γ on the expression of IRF-1 and iNOS (A). Total RNAs were isolated from ESTDAB-159 cell line and form the control cell line ESTDAB-056 grown in the presence or absence of 800 U/ml IFN-γ for the indicated periods of time and analyzed for the relative levels of mRNA by RT-PCR.
Techniques Used: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction
Figure Legend Snippet: EMSA showing the binding of IRF-1 to a 32 P labeled probe containing the IRF-1 consensus sequence. EMSA was performed with nuclear extracts obtained from untreated ESTDAB-056 (3) and ESTDAB-159 (5) melanoma cells or treated with 800 U/ml IFN-γ for 4 h (lanes 1, 4 and 7 corresponding to ESTDAB-056; and lanes 2, 6 and 8 corresponding to ESTDAB-159). A 50-fold molar excess of unlabeled IRF-1 probe was added to the binding reaction (lane 1, 2) to compete out the formation of a detectable complex. Anti-IRF-1 antibody was used to block IRF-1 binding to test the specificity of the interaction (lanes 7 and 8). Results shown are representative of at least three independent experiments.
Techniques Used: Binding Assay, Labeling, Sequencing, Blocking Assay
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